Interactions of CT-DNA with Hypocrellin A and its Al³⁺-Hypocrellin A complex

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• 作者：Fei Ma ; Xuefeng Ge ; Heyong Huang ; Chao Yang ; Lina Han ; Jiahong Zhou ; ^{zhoujiahong@njnu.edu.cn} ; Xiaodi Yang ; ^{yangxiaodi@njnu.edu.cn}
• 关键词：Hypocrellin A ; Al3+&ndash ; Hypocrellin A complex ; Calf thymus DNA ; UV&ndash ; vis spectroscopy ; Fluorescence spectra ; Circular dichroism spectroscopy
• 刊名：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
• 出版年：2013
• 出版时间：15 May, 2013
• 年：2013
• 卷：109
• 期：Complete
• 页码：158-163
• 全文大小：716 K

文摘

In this study, the chelation of Hypocrellin A (HA) with Al³⁺ in water solution has been synthesized, and the interactions of HA and Al³⁺-HA complex with calf thymus DNA are in detail compared by UV-vis and fluorescence spectroscopic techniques, circular dichroism spectroscopy and viscosity measurement. The experiment results suggest that HA and Al³⁺-HA complex both could bind to CT DNA by intercalation mode, and double helix of DNA was damaged. Moreover, Al³⁺-HA complex not only displays higher absorption at therapeutic window but also displays stronger binding affinity to CT DNA than HA.