Small molecules enhance functional O-mannosylation of Alpha-dystroglycan

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• 作者：Fengping Lv^a ; ^&dagger ; ; Zhi-fang Li^b ; ^c ; ^&dagger ; ; Wenhao Hu^a ; ^{whu@chem.ecnu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Xiaohua Wu^b ; ^c ; ^{xwu@mannanpharma.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Dystroglycan ; Dystroglycanopathy ; O-Mannosylation ; Cell-based high-throughput screening
• 刊名：Bioorganic & Medicinal Chemistry
• 出版年：2015
• 出版时间：15 December 2015
• 年：2015
• 卷：23
• 期：24
• 页码：7661-7670
• 全文大小：9509 K

文摘

Alpha-dystroglycan (α-DG), a highly glycosylated receptor for extracellular matrix proteins, plays a critical role in many biological processes. Hypoglycosylation of α-DG results in various types of muscular dystrophies and is also highly associated with progression of majority of cancers. Currently, there are no effective treatments for those devastating diseases. Enhancing functional O-mannosyl glycans (FOG) of α-DG on the cell surfaces is a potential approach to address this unmet challenge. Based on the hypothesis that the cells can up-regulate FOG of α-DG in response to certain chemical stimuli, we developed a cell-based high-throughput screening (HTS) platform for searching chemical enhancers of FOG of α-DG from a large chemical library with 364,168 compounds. Sequential validation of the hits from a primary screening campaign and chemical works led to identification of a cluster of compounds that positively modulate FOG of α-DG on various cell surfaces including patient-derived myoblasts. These compounds enhance FOG of α-DG by almost ten folds, which provide us powerful tools for O-mannosylation studies and potential starting points for the development of drug to treat dystroglycanopathy.