Characterization of the single cycle replication of HIV-1 expressing Gaussia luciferase in human PBMCs, macrophages, and in CD4+ T cell-grafted nude mouse
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- 作者：Zhujun Aoa ; c ; Jing Huangb ; Xiaoli Tanb ; Xiaoxia Wanga ; 1 ; Tian Tianb ; Xu Zhanga ; Qingjian Ouyangb ; Xiaojian Yaoa ; b ; xiao-jian.yao@umanitoba.ca" class="auth_mail" title="E-mail the corresponding author
- 关键词：HIV ; Single cycle infection ; Gaussia luciferase ; CD4+ lymphocytes ; Macrophages ; CD4+ T cell drafted nude mouse
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：228
- 期：Complete
- 页码：95-102
- 全文大小：1548 K
文摘
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HIV-1 single cycle replicating virus expresses a secreted Gaussia luciferase (Gluc).
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Have deletions at RT, IN and Env genes and no wild type virus conversion.
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Efficiently infected human primary cells and CD4+ T cell-grafted nude mouse.
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Monitory of Gluc activity is simple and more sensitive than anti-p24 ELISA assay.
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A safe and valuable HIV-1 infection system for biosafety level-2 laboratory.