Development and validation of a sensitive UHPLC-MS/MS method for quantitation of prucalopride in rat plasma and its application to pharmacokinetics study
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- 作者：Zhi Sun1 ; Lihua Zuo1 ; Jian Kang ; Lin Zhou ; Mengmeng Jia ; Zeyun Li ; Zhiheng Yang ; Xiaojian Zhang ; zhangxiaojian_yxb@163.com" class="auth_mail" title="E-mail the corresponding author ; Zhenfeng Zhu ; zhenfeng1997@sina.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：UHPLC&ndash ; MS/MS ; ultra high performance liquid chromatography with tandem mass spectrometry ; IS ; internal standard ; ESI ; electrospray ionization ; LLOQ ; the lower limit of quantitation ; RE ; relative error ; RSD ; relative standard deviation ; CE ; collision energy ; MRM ; multiple reaction monitoring
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：15 October 2016
- 年：2016
- 卷：1033-1034
- 期：Complete
- 页码：328-333
- 全文大小：878 K
文摘
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Developing and verifying of a high precision and accuracy UHPLC–MS/MS method for the determination of prucalopride in rat plasma.
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The comparative pharmacokinetic study of prucalopride following oral administration of different dosages to female and male rats is for the first time reported.
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This selective and sensitive method (LLOQ = 100 pg/mL) requires quite low plasma sample volume (100 μL).
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Extraction performed with a simple and effective protein precipitation preparation procedure for prucalopride.