Design a chemically defined/medically approved medium for cell transplantation according to the metabolic characteristics of microencapsulated cells and the process of encapsulation

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• 作者：Guojun Lv ; Zhijie Sun ; Na Li ; Shuangyue Li ; Ying Zhang ; Yubing Xie ; Weiting Yu ; Wei Wang ; Xiaojun Ma
• 关键词：Cell transplantation ; Microencapsulation ; Recombinant CHO cell ; Chemically defined medium ; Amino acid
• 刊名：Biochemical Engineering Journal
• 出版年：2008
• 出版时间：1 April 2008
• 年：2008
• 卷：39
• 期：1
• 页码：157-163
• 全文大小：431 K

文摘

Cell microencapsulation is a promising therapeutic strategy to many diseases; however, the metabolic characteristics of microencapsulated cells and also the optimum microcapsule additives that meet the criteria for long-term functional immunoisolated transplantation have rarely been taken into consideration hitherto. In this work, the optimized amino acid supplements and several chemically defined compounds were applied to cultivation of the microencapsulated CHO cells. The results show that the optimized medium significantly enhances cell survival and, particularly, endostatin secretion of encapsulated CHO cells over a time period of 8 weeks. Furthermore, long-term in vitro stability of alginate–poly-l-lysine–alginate (APA) microcapsules underwent no changes, which indicated that this optimized medium might be feasible to maintain high viability of microencapsulated cell for short periods of time before implantation.