53 Magnesium sulfate affords neuroprotection in an eclampsia-like model induced in pregnant rats by ameliorating brain edema and neuroinflammation: Immune and inflammatory mechanisms

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• 作者：Xiaolan Li ; Xinjia Han ; Huishu Liu
• 刊名：Pregnancy Hypertension: An International Journal of Women's Cardiovascular Health
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：6
• 期：3
• 页码：162
• 全文大小：38 K

文摘

Eclampsia is defined as the onset of grand mal seizures on the basis of preeclampsia. Presently, magnesium sulfate (MgSO₄) is the most effective drug in treating elcamptic seizure, but the mechanism how (MgSO₄) prevents eclampsia is still unclear.

Objectives

The aim of the present study was to investigate the neuroprotective effects of (MgSO₄) on an eclampsia-like seizure model and explore the related mechanism.

Methods

We investigated the effects of (MgSO₄) on brain edema, neuronal loss and neuroinflammation following pentylenetetrazol-induced eclampsia-like seizure in a rat model of preeclampsia. Brain water content was measured for assessing brain edema; enzyme-linked immunosorbent assay was used for detecting the cerebrospinal fluid levels of S100-B and Ferritin which are associated with neuroinflammation; nissl staining was used for detecting neuronal loss in the hippocampal CA1 region (CA1), immunofluorescence staining of Iba-1 and GFAP was used for detecting the density of microglia and astrocytes in the CA1 area.

Results

Our results revealed that (MgSO₄) effectively attenuated brain edema, and significantly decreased the cerebrospinal fluid levels of S100-B and Ferritin; Furthermore, MgSO₄ statistically ameliorated neuronal loss in the hippocampal CA1 area and inhibited the increase of microglia and astrocytes following eclampsia-like seizure.

Conclusions

Magnesium sulfate administration prevented neuronal loss in our rat model of eclampsia possiblely by causing a reduction in brain edema and neuroinflammation.