Highly sensitive enzyme-free immunosorbent assay for porcine circovirus type 2 antibody using Au-Pt/SiO2 nanocomposites as labels
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- 作者：Long Wu1 ; Wenmin Yin1 ; Kun Tang ; Kang Shao ; Qin Li ; Pan Wang ; Yunpeng Zuo ; Xiaomin Lei ; Zhicheng Lu ; Heyou Han ; hyhan@mail.hzau.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Enzyme-free immunosorbent assay ; Au-Pt/SiO2 nanocomposites ; Fe3O4 magnetic beads ; Human IgG ; Porcine circovirus type 2
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 August 2016
- 年：2016
- 卷：82
- 期：Complete
- 页码：177-184
- 全文大小：2550 K
文摘
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An enzyme-free ELISA system was developed for cost-effective monitoring of PCV2.
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Au-Pt/SiO2 nanoparticles took place of HRP with lower cost, easier preparation and better peroxidase-like activity.
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F3O4 magnetic beads were utilized to entrap the substrate for convenient separation.
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The system behaved comparable sensitivity with conventional ELISA even in the absence of H2O2.
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The system exhibited a 264-fold improvement in HIgG detection as compared with conventional ELISA.