Essential RNA-Based Technologies and Their Applications in Plant Functional Genomics

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• 作者：Sachin Teotia¹ ; ² ; ³ ; Deepali Singh² ; ³ ; Xiaoqing Tang³ ; Guiliang Tang¹ ; ³ ; ^{gtang1@mtu.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：target mimic (TM) ; short tandem target mimic (STTM) ; microRNA sponge (SP) ; artificial microRNA (amiRNA) ; artificial/synthetic tasiRNA (atasiRNA/syn-tasiRNA) ; CRISPR/Cas9
• 刊名：Trends in Biotechnology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：34
• 期：2
• 页码：106-123
• 全文大小：3299 K

文摘

Genome sequencing has not only extended our understanding of the blueprints of many plant species but has also revealed the secrets of coding and non-coding genes. We present here a brief introduction to and personal account of key RNA-based technologies, as well as their development and applications for functional genomics of plant coding and non-coding genes, with a focus on short tandem target mimics (STTMs), artificial microRNAs (amiRNAs), and CRISPR/Cas9. In addition, their use in multiplex technologies for the functional dissection of gene networks is discussed.