Secretory expression of a heterologous protein, Aiio-AIO6BS, in Bacillus subtilis via a non-classical secretion pathway
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- 作者：Xingliang Pan ; Yalin Yang ; Xuewei Liu ; Dong Li ; Juan Li ; Xiaoze Guo ; Zhigang Zhou ; zhouzhigang03@caas.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：AIO6BS ; Secretion ; Non-classical export pathway ; Bacillus subtilis ; Cell lysis
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2016
- 出版时间：16 September 2016
- 年：2016
- 卷：478
- 期：2
- 页码：881-886
- 全文大小：886 K
文摘
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Seven (7) N-terminus residues and 3 C-terminus residues of the AIO6BS are required for its secretion.
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Glu7 mutations within AIO6BS have no impact on protein secretion.
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The cys267 or hydrophobic residue of the AIO6BS is important for protein expression and secretion in Bacillus subtilis.