Functional characterisation of a tropine-forming reductase gene from Brugmansia arborea, a woody plant species producing tropane alkaloids
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- 作者：Wei Qianga ; Ke Xiaa ; Qiaozhuo Zhanga ; Junlan Zenga ; Yuanshe Huangb ; Chunxian Yanga ; Min Chenc ; Xiaoqiang Liua ; Xiaozhong Land ; Zhihua Liaoa ; zhliao@swu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; zhihualiao@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Brugmansia arborea ; Solanaceae ; Tropane alkaloids ; Tropinone reductase I ; Enzymatic kinetics ; Tissue profile
- 刊名：Phytochemistry
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：127
- 期：Complete
- 页码：12-22
- 全文大小：2651 K
文摘
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Brugmansia arborea is a woody plant species predominantly producing scopolamine.
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The expression profile of BaTRI showed no root specificity.
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BaTRI catalysed tropinone reduction at pH 6.4 and tropine oxidation at pH 9.6.
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BaTRI had higher tropinone reduction efficiency than DsTRI.