Detection of serum β₂-GPI–Lp(a) complexes in patients with systemic lupus erythematosus

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• 作者：Chunni Zhang ; Ke Li ; Beilei Shi ; Xiangdong Wang ; Xiaozhuan Liu ; Weisong Qin ; Aizhong Han ; Junjun Wang
• 关键词：Lipoprotein(a) ; Oxidative modification ; β ; ₂-glycoprotein-I ; Enzyme-linked immunosorbent assay ; Oxidative stress ; Atherosclerosis ; Risk factor
• 刊名：Clinica Chimica Acta
• 出版年：2010
• 出版时间：2 March 2010
• 年：2010
• 卷：411
• 期：5-6
• 页码：395-399
• 全文大小：298 K

文摘

Background

Circulating β₂-glycoprotein-I-oxidized low-density lipoprotein (β₂-GPI–ox-LDL) complexes have been found in patients with systemic lupus erythematosus (SLE) and other autoimmune diseases as a contributor to the development of autoimmune-mediated atherosclerosis. In vitro study showed that β₂-GPI also bound with high affinity to atherogenic lipoprotein (a) [Lp(a)] which shares structural similarity to LDL. We examined the existence and clinical significance of serum complexes of β₂-GPI with Lp(a) in SLE patients.

Methods

A “sandwich” ELISA was developed for measuring serum concentrations of β₂-GPI–Lp(a) complexes, using rabbit anti-human β₂-GPI antibody as capturing antibody, and quantitating with antibody against apo(a). Forty-seven SLE patients and 42 healthy controls were studied.

Results

Both Lp(a) (400 ± 213 mg/l vs. 181 ± 70 mg/l) and ox-Lp(a) (27.07 ± 22.30 mg/l vs. 8.20 ± 4.55 mg/l) concentrations were higher in SLE patients than in controls (P < 0.0001). β₂-GPI–Lp(a) complexes were detectable in both controls and SLE. The complexes levels in SLE were higher than in controls (0.96 ± 0.41 U/ml vs. 0.59 ± 0.20 U/ml, P < 0.0001) and was positively correlated with ox-Lp(a) (P < 0.001).

Conclusions

We report the existence of β₂-GPI–Lp(a) complexes in both controls and SLE patients. The complexes levels increase in SLE.