Structural elucidation of the polysaccharide moiety of a glycopeptide (GLPCW-II) from Ganoderma lucidum fruiting bodies

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• 作者：LiBin Ye ; JingSong Zhang ; XiJun Ye ; QingJiu Tang ; YanFang Liu ; ChunYu Gong ; XiuJui Du ; YingJie Pan
• 关键词：Ganoderma lucidum ; Glycopeptide ; NMR spectroscopy ; Polysaccharide moiety ; Structural analysis
• 刊名：Carbohydrate Research
• 出版年：2008
• 出版时间：17 March 2008
• 年：2008
• 卷：343
• 期：4
• 页码：746-752
• 全文大小：294 K

文摘

A water-soluble glycopeptide (GLPCW-II) was isolated from the fruiting bodies of Ganoderma lucidum by DEAE-Sepharose Fast-Flow and Sephacryl S-300 High Resolution Chromatography. The glycopeptide had a molecular weight of 1.2 × 10⁴ Da (determined by HPLC), and consisted of 90 % carbohydrate and 8 % protein as determined using the phenol-sulfuric acid method and the BCA^TM protein assay reagent kit, respectively. The polysaccharide moiety was composed mainly of pan class=""smCaps"">dpan>-Glc, pan class=""smCaps"">lpan>-Fuc, and pan class=""smCaps"">dpan>-Gal in the ratio of 1.00:1.09:4.09. To facilitate structure–activity studies, the structure of the GLPCW-II polysaccharide moiety was elucidated using ¹H and ¹³C NMR spectroscopy including COSY, TOCSY, HMBC, HSQC, and ROESY, combined with GC–MS of methylated derivatives, and shown to consist of repeating units with the following structure: