Angelica sinensis polysaccharide nanoparticles as novel non-viral carriers for gene delivery to mesenchymal stem cells

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• 作者：Wenwen Deng ; Min Fu ; Yue Cao ; Xia Cao ; Miao Wang ; Yan Yang ; Rui Qu ; Jun Li ; Ximing Xu ; Jiangnan Yu
• 关键词：Nanoparticle ; Polysaccharide ; Non-viral vector ; Polyethylenimine ; Mesenchymal stem cells
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：November, 2013
• 年：2013
• 卷：9
• 期：8
• 页码：1181-1191
• 全文大小：1743 K

文摘

This study centers on the use of a nanoparticle based on the polysaccharide from Angelica sinensis (ASP) as an efficient and safe non-viral gene vector. After modification with branched low molecular weight polyethylenimine (1200 Da), the cationized ASP (cASP) was combined with the plasmid encoding transforming growth factor-beta 1 (TGF-尾1) to form a spherical nano-scaled particle (i.e., cASP-pTGF-尾1 nanoparticle). This nanoparticle was applied to transfect rat bone marrow mesenchymal stem cells and human umbilical cord mesenchymal stem cells. As a result, nanoparticles (cASP/pDNA weight ratio 10:1) had the greatest transfection efficiency in both cells, which was significantly higher than those of Lipofectamine2000 and PEI (25 kDa). This was in agreement with the findings of the semi-quantitative RT-PCR and live cell imaging. These nanoparticles were also less toxic than Lipofectamine2000 and PEI (25 kDa). Therefore, cASP could be a potential candidate for a novel non-viral gene vector.

From the Clinical Editor

These authors demonstrate the use of a nanoparticle-based efficient and safe non-viral gene vector delivery system via a spherical nanoparticle based on a polysaccharide from Angelica sinensis, with parameters superior to Lipofectamine2000.