Intermediate-sensor assisted push-pull strategy and its application in heterologous deoxyviolacein production in Escherichia coli
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- 作者：Mingyue Fanga ; 1 ; Tianmin Wanga ; 1 ; Chong Zhanga ; chongzhang@tsinghua.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Jili Baia ; Xiang Zhenga ; Xuejin Zhaob ; Chunbo Loub ; Xin-Hui Xinga
- 关键词：Pathway engineering ; High-throughput screening ; Biosensor ; Deoxyviolacein biosynthesis ; Sequential optimization
- 刊名：Metabolic Engineering
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：33
- 期：Complete
- 页码：41-51
- 全文大小：1691 K
文摘
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An L-Trp biosensor was developed based on native control of the tnaCAB operon.
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Deoxyviolacein pathway was divided into two parts, connected by an intermediate.
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The senor was used for screening upstream module library based on enhanced output.
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Screening downstream enzyme mutant library was based on reduced sensor output
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With this push/pull approach, 1.92g/L deoxyviolacein titer was obtained.