Response of the hepatic transcriptome to aflatoxin B₁ in ducklings

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• 作者：Ni-Ya Zhang^a ; Ming Qi^a ; Xin Gao^a ; Ling Zhao^a ; Jie Liu^a ; Chang-Qin Gu^b ; Wen-Jing Song^a ; Christopher Steven Krumm^c ; Lv-Hui Sun^a ; ^{lvhuisun@mail.hzau.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; De-Sheng Qi^a ; ^{qds@mail.hzau.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Aflatoxin B1 ; Duckling ; Liver ; Gene expression ; RNA-Seq
• 刊名：Toxicon
• 出版年：2016
• 出版时间：1 March 2016
• 年：2016
• 卷：111
• 期：Complete
• 页码：69-76
• 全文大小：1431 K

文摘

This study was conducted to determine the effects of aflatoxin B₁ (AFB₁) on the hepatic transcriptome in ducklings through RNA-sequencing (RNA-Seq). Twenty four, 1-day-old ducklings were divided into 4 treatment groups. Each group received an oral dose of AFB₁ at 0, 10, 20, 40 μg/kg BW per day for 2 weeks. Administration of 20 and 40 μg/kg BW of AFB₁ significantly decreased body weight, feed intake, serum total protein and albumin, while increasing serum aspartate aminotransferase and alanine aminotransferase activities, and hepatic histopathological lesions. Furthermore, RNA was extracted from the liver of ducklings administrated 0 and 40 μg/kg BW of AFB₁. Two RNA-Seq libraries were created from pooled samples and produced over 149 M reads, totaling 14.9 Gb of sequence. Approximately 96,953 predicted transcripts were assembled, 749 of which had significant differential expressions (≥2-fold) between the control and AFB₁ treatment. GO and KEGG pathway analysis results showed that many genes involved in phase I metabolism, phase II detoxification, oxidation-reduction process, carcinogenesis, apoptosis and cell cycle, and fatty acid metabolism were affected by AFB₁ exposure. Conclusion, this study determined the hepatic transcriptome responded to AFB₁ exposure, and provide candidate genes can be targeted to prevent and/or reduce aflatoxicosis in ducklings.