Pulsed light (PL) inactivation of viruses and bacteria were evaluated.
Complete inactivation of viruses and bacteria can be achieved within seconds in PBS.
MNV-1 and Tulane virus are more PL-resistant than Salmonella and Escherichia coli O157:H7.
PL treatment at fluence level <12 J/cm2 cannot completely eliminate targeting organisms on berries.