Analysis of urinary metabolites for breast cancer patients receiving chemotherapy by CE-MS coupled with on-line concentration

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• 作者：Lanfang Yu ; Cheng Jiang ; Sui Huang ; Xingchu Gong ; Shufang Wang ; Peng Shen
• 关键词：CE-MS ; Organic acid ; Amino acid ; Urine ; Breast cancer
• 刊名：Clinical Biochemistry
• 出版年：2013
• 出版时间：August, 2013
• 年：2013
• 卷：46
• 期：12
• 页码：1065-1073
• 全文大小：1078 K

文摘

Objective

This study aimed to explore the relationship between urinary metabolites and clinical chemotherapy response in breast cancer by CE-MS coupled with on-line concentration.

Design and methods

Urine samples were obtained from patients with advanced or locally advanced breast cancer (n = 21) before and after chemotherapy and healthy volunteers (n = 21). A rapid and sensitive hexadimethrine bromide-coating CE-MS method coupled with normal stacking is developed for the determination of organic acids in human urine. Another CE-MS method coupled with pH-mediated sample stacking is used for the analysis of amino acids and organic acids.

Results

After receiving chemotherapy, chemotherapy-sensitive patients showed 30 % change in metabolite levels compared to healthy people, while chemotherapy-insensitive patients showed only 9 % change in metabolite levels compared to healthy people showing recurrence. The extent of energy insufficiency for chemotherapy-insensitive patients was greater than that for chemotherapy-sensitive patients.

Conclusions

Urinary metabolic products may be new potential predictive markers for therapy efficacy. However, more studies with a larger sample size are required to confirm these conclusions.