Effect of Feining on bleomycin-induced pulmonary injuries in rats

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• 作者：Xiangyan Liang^a ; ¹ ; Qiong Tian^b ; ^{tqiong@fmmu.edu.cn} ; Zhangrui Wei^b ; ¹ ; Fang’ ; e Liu^a ; Jiankang Chen^a ; Yufeng Zhao^a ; Ping Qu^a ; Xiaojun Huang^a ; Xingchun Zhou^a ; Nannan Liu^a ; Fei Tian^a ; Ru Tie^a ; Libing Liu^a ; Jun Yu^a ; ¹ ; ^{yujunby@fmmu.edu.cn}
• 关键词：Feining ; Gentiopicroside ; Panax notoginseng saponins ; Pulmonary alveolitis ; Bleomycin
• 刊名：Journal of Ethnopharmacology
• 出版年：2011
• 出版时间：12 April 2011
• 年：2011
• 卷：134
• 期：3
• 页码：971-976
• 全文大小：584 K

文摘

Ethnopharmacological relevance

The flowers of Gentiana veitchiorum has been widely used in decoction form in the traditional medicine of Tibet against tussis, tracheitis, angina for their anti-inflammatory, antimicrobial and alexipharmic properties.

Aim of the study

The aim of current study was to evaluate the therapeutic effects of Feining, a Chinese herbal formula (national invention patent: ZL200510042636.3) against pulmonary injuries and to clarify the mechanisms involved.

Materials and methods

Experimental pulmonary injuries were induced by bleomycin (BLM) in rats with or without subsequent treatment of Feining or prednisone as positive control. The pulmonary injuries were evaluated by histological analysis. Also, the levels of superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH) and hydroxyproline (Hyp) in the lung tissue were determined. To clarify one of the possible active principles responsible for Feining, high performance liquid chromatography–diode array detector–mass spectrometry (HPLC–DAD–MS) method was applied to identify the components of Gentiana veitchiorum, one of major ingredients of Feining.

Results

Feining significantly improved lung alveolitis scores and reduced the Hyp content of lungs, which is an index of collagen accumulation. Moreover, Feining played a role against the oxidative damages by decreasing the MDA level, whereas increasing SOD and GSH activity, which correlated with oxidation resistance and scavenging of free radicals. In addition, Feining alleviated inflammatory lung injury by decreasing tumor necrosis factor-α (TNF-α) expression. HPLC–DAD–MS analysis revealed that there was 1.97 % gentiopicroside in Gentiana veitchiorum.

Conclusion

Feining has certain therapeutic effects against pulmonary injuries.