Nanoparticle-based adjuvant for enhanced protective efficacy of DNA vaccine Ag85A-ESAT-6-IL-21 against Mycobacterium tuberculosis infection

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• 作者：Fangliu Yu ; PhD^a ; ¹ ; Jing Wang ; BS^b ; ¹ ; Jun Dou ; PhD^a ; ¹ ; ^{njdoujun@yahoo.com.cn} ; Haitao Yang ; PhD^c ; Xingfeng He ; PhD^a ; Weiguo Xu ; BS^c ; Yu Zhang ; PhD^d ; Kai Hu ; PhD^a ; Ning Gu ; PhD^d
• 关键词：Mycobacterium tuberculosis ; Nanoparticles ; Interleukin-21 ; DNA vaccine
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2012
• 出版时间：November, 2012
• 年：2012
• 卷：8
• 期：8
• 页码：1337-1344
• 全文大小：1140 K

文摘

The goal of this study was to evaluate the protective efficacy of a cationic nanoparticle-based DNA vaccine expressing antigen 85A (Ag85A) and 6-kDa early secretory antigen target (ESAT-6) of Mycobacterium tuberculosis as well as cytokine interleukin-21 (IL-21) against M. tuberculosis infection. The results of this indicated that the anti-M. tuberculosis immune responses were induced in mice that had received the different DNA vaccines. More importantly, compared with using DNA vaccine Ag85A-ESAT-6-IL-21 alone, the nanoparticle-based DNA vaccine Ag85A-ESAT-6-IL-21 showed a statistically significant increase in the protective efficacy against M. tuberculosis infection in the immunized mice. We concluded that the nanoparticle-based DNA vaccine induced a strong immune response and markedly inhibited the growth of the M. tuberculosis in the mice. These findings highlighted the potential utility of Fe₃O₄-Glu-polyethyleneimine nanoparticles encapsulated with the DNA vaccine as a prophylactic vaccine in the M. tuberculosis-infected mouse model.

From the Clinical Editor

This study emphasizes the potential utility of Fe₃O₄-Glu-polyethyleneimine nanoparticles encapsulated with DNA vaccine against TB as a prophylactic vaccine. The authors demonstrated a strong immune response and marked growth inhibition of mycobacterium tuberculosis in the mice.