Polyvalent display of RGD motifs on turnip yellow mosaic virus for enhanced stem cell adhesion and spreading

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• 作者：Xingjie Zan ; Pongkwan Sitasuwan ; Joshua Powell ; Theo W. Dreher ; Qian Wang
• 关键词：Virus ; RGD insertion ; Layer-by-layer assembly ; Stem cell ; Turnip yellow mosaic virus
• 刊名：Acta Biomaterialia
• 出版年：2012
• 出版时间：August, 2012
• 年：2012
• 卷：8
• 期：8
• 页码：2978-2985
• 全文大小：1883 K

文摘

Turnip yellow mosaic virus (TYMV) is a stable 28 nm icosahedral plant virus that can be isolated in gram quantities. In order to study the polyvalent effect of Arg-Gly-Asp (RGD) clustering on the response of bone marrow stem cells (BMSCs), an RGD motif was genetically displayed on the coat protein of the TYMV capsid. Composite films composed of either wild-type TYMV or TYMV-RGD44, in combination with poly(allylamine hydrochloride) (PAH), were fabricated by a layer-by-layer adsorption of virus and PAH. The deposition process was studied by quartz crystal microbalance, UV-visible spectroscopy and atomic force microscopy. BMSC adhesion assays showed enhanced cell adhesion and spreading on TYMV-RGD44 coated substrates compared to native TYMV. These results demonstrate the potential of TYMV as a viable scaffold for bioactive peptide display and cell culturing studies.