Functional analysis of the venom allergen-like protein gene from pine wood nematode Bursaphelenchus xylophilus using a baculovirus expression system
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- 作者：Yong-Xia Lia ; b ; Ying Wanga ; b ; Zhen-Yu Liua ; c ; Xuan Wanga ; b ; Quan Lua ; b ; Xiu-Zhen Jiaa ; b ; Xing-Yao Zhanga ; b ; zhangxingyao@126.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Bursaphelenchus xylophilus ; Pinus massoniana ; Venom allergen-like protein ; Insect cell expression ; α-Pinene synthetase gene
- 刊名：Physiological and Molecular Plant Pathology
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：93
- 期：Complete
- 页码：58-66
- 全文大小：2042 K
文摘
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Recombinant venom allergen-like protein (VAP) was obtained and by expressing Bx-vap-1 in insect cells.
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Three-year-old Pinus massoniana were inoculated with recombinant VAP, simulating B. xylophilus esophageal gland secretions.
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We examine the changes of the α-pinene synthase gene expression and α-terpene content of P. massoniana after inoculation.
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We detected the pathological, Cytological and cavitation changes of the pine branch 15d post inoculation.
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The recombinant VAP could damage P. massoniana cells. Recombinant VAP did not, however, lead to cavitation.