MicroRNA-4262 activates the NF-κB and enhances the proliferation of hepatocellular carcinoma cells

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• 作者：Sen Lu^a ; Jindao Wu^a ; Yuanyuan Gao^b ; Guoyong Han^a ; Wenzhou Ding^a ; Xinli Huang^a ; ^{huangxinli0118@126.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：miR-4262 ; PDCD4 ; Hepatocellular carcinoma
• 刊名：International Journal of Biological Macromolecules
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：86
• 期：Complete
• 页码：43-49
• 全文大小：2214 K

文摘

microRNAs (miRNAs) were known as transcriptional regulators to regulate the repertoires of target genes during the development of hepatocellular carcinoma (HCC). In this study, we investigated the roles of miR-4262 in the process of HCC. Our results showed that miR-4262 is overexpressed in HCC tissues and hepatoma cell lines (HepG2 and Sk-hep-1). We also found that miR-4262 enhanced the process of cell cycle and inhibited the apoptosis leading to promoted cell proliferation and activity. Moreover, the 3′UTR of PDCD4 was complementary to miR-4262 seed region and we confirmed that PDCD4 is the direct target of miR-4262 with 3′UTR luciferase assay. qPCR and WB analysis revealed that the level of PDCD4 was negatively correlated with the expression of miR-4262 in HepG2 cells. Furthermore, our results demonstrated that miR-4262-promoted cell proliferation was mediated by PDCD4 and miR-4262 can activate the NF-κB pathway to promote the accumulation of nuclear NF-κB/P65. All of these findings suggested miR-4262 may play a role in the development of HCC.