- 作者：Bei Bei DONG ; Cui Ling XU ; Li Bo DONG ; Hui Jian CHENG ; Lei YANG ; Shu Mei ZOU ; Min CHEN ; Tian BAI ; Ye ZHANG ; Rong Bao GAO ; Xiao Dan LI ; Jing Hong SHI ; Hui YUAN ; Jing YANG ; Tao CHEN ; Yun ZHU ; Ying XIONG ; Shuai YANG ; Yue Long SHU
- 关键词：Avian influenza ; H3N8 ; Sequence analysis
- 刊名：Biomedical and Environmental Sciences
- 出版年：2013
- 出版时间：July, 2013
- 年：2013
- 卷：26
- 期：7
- 页码：546-551
- 全文大小：4729 K
Objective
To conduct a full genome sequence analysis for genetic characterization of an H3N8 influenza virus isolated from drinking water of a domestic duck farm in Poyang Lake area in 2011.Methods
The virus was cultivated by specific pathogen free (SPF) chicken embryo eggs and was subtyped into hemagglutinin (HA) and neuraminidase (NA) by real-time PCR method. Eight gene segments were sequenced and phylogenetic analysis was conducted.
Results
The NA gene of this virus belongs to North American lineage; other seven genes belong to Eurasian lineage. Compared with the viruses containing NA gene, the PB2 and PB1 gene came from different clades. And this indicates that the virus was a novel reassortant genotype. The HA receptor binding preference was avian-like and the cleavage site sequence showed a low pathogenic feature. There was no drug resistance mutation of M2 protein. The mutations of Asn30Asp, and Thr215Ala of the M1 protein implied the potential of pathogenicity increase in mice.
Conclusion
The finding of novel genotype of H3N8 virus in drinking water in this duck farm near Poyang Lake highlighted the importance of strengthening the surveillance of avian influenza in this region, which could contribute to pinpointing the influenza ecological relations among avian, swine, and human.