Neurotoxic effect of subacute benzo(a)pyrene exposure on gene and protein expression in Sprague-Dawley rats
详细信息 查看全文
- 作者：Shu-qun Cheng ; Yin-yin Xia ; Jun-lin He ; Xue-qing Liu ; Xue-mei Chen ; Yu-bin Ding ; Ying-xiong Wang ; Bing Peng ; Bai-jie Tu
- 关键词：Benz[a]pyrene ; Rat ; Hippocampus ; Water maze ; Microarray
- 刊名：Environmental Toxicology and Pharmacology
- 出版年：2013
- 出版时间：September, 2013
- 年：2013
- 卷：36
- 期：2
- 页码：648-658
- 全文大小：1924 K
文摘
Benzo(a)pyrene (B[a]P) is an environmental carcinogen that induces tumors in many animal species, but the neurotoxic effects of B[a]P have not been well studied. In the present study, we investigated the effects of subacute exposure to B[a]P in Sprague-Dawley (SD) rats. Male rats received daily injections of either B[a]P (0, 1, 2.5, or 6.25 mg/kg, i.p.) or vehicle for 45 days. Exposure to B[a]P affected the behavior of rats in the Morris water maze test. Gene microarray and real-time PCR analyses revealed that exposure to B[a]P affected signal transduction in the rat hippocampus. Protein microarray analysis revealed that altered protein expression played a role in cell death in the functional annotation cluster analysis. Finally, major vault protein was found to display low cDNA and protein expression levels. The present study explored some of the possible mechanisms underlying B[a]P neurotoxicity and provided evidence that B[a]P plays a neurotoxic role in rats.