Purification, characterization and biological activity on hepatocytes of a polysaccharide from Flammulina velutipes mycelium

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• 作者：Xiubing Pang ; Wenbing Yao ; Xiaobing Yang ; Chen Xie ; Dong Liu ; Jian Zhang ; Xiangdong Gao
• 关键词：Flammulina velutipes ; Polysaccharide ; Mycelium ; Structure ; d-Glucan ; Hepatocyte
• 刊名：Carbohydrate Polymers
• 出版年：2007
• 出版时间：1 October 2007
• 年：2007
• 卷：70
• 期：3
• 页码：291-297
• 全文大小：240 K

文摘

A water-soluble polysaccharide named as FVP2 has been isolated from Flammulina velutipes mycelium by hot water extraction, anion-exchange and gel-permeation chromatography. Structure study shows FVP2 is an α-(1→4)-d-glucan, with a single α-d-glucan at the C-6 position approximately every seven residues, along the main chain. The weight-average molecular weight is 1.89 × 10⁴ Da. Its biological activity was tested on hepatocytes. In vitro study indicates FVP2 can enhance the livability of primary culture of mouse hepatocytes and decrease the release of ALT as well as apoptosis of hepatocytes after CCl₄ intoxication.