Expression, purification and characterization of a functional, recombinant, cold-active lipase (LipA) from psychrotrophic Yersinia enterocolitica
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- 作者：Xiuling Jia ; Shan Lia ; Baoqiang Wangb ; Qi Zhanga ; Lianbing Lina ; Zhiyang Dongc ; Yunlin Weia ; weiyunlin18@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：E. coli ; Escherichia coli ; SDS&ndash ; PAGE ; sodium dodecyl sulfate&ndash ; polyacrylamide gel electrophoresis ; p-NPB ; p-nitrophenylbutyrate ; IPTG ; isopropyl 尾-d-1-thiogalactopyranoside ; EDTA ; ethylenediaminetetraacetic acid ; BGL ; Burkholderia glumae lipase ; BCL ; B. cepacia lipase ; PAL ; Pseudomonas aeruginosa lipae ; PML ; Proteus mirabilis lipase ; YEL ; Y. enterocolitica strain KM1 lipase ; DMSO ; dimethyl sulfoxide
- 刊名：Protein Expression and Purification
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：115
- 期：Complete
- 页码：125-131
- 全文大小：2152 K
文摘
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We report the cloning and heterogonous soluble expression of a novel cold-active lipase gene.
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The effects of temperature, pH, metal ions and organic solvent on LipA activity were examined.
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Sequence alignment and phylogenetic analysis showed this cold-active LipA is a new member of bacterial lipase family I.1.