Optimized dissociation protocol for isolating human glioma stem cells from tumorspheres via fluorescence-activated cell sorting
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- 作者：Donglai Lva ; b ; 1 ; Qing-hua Maa ; b ; 1 ; Jiang-jie Duana ; b ; Hai-bo Wua ; b ; Xi-long Zhaoa ; b ; Shi-cang Yua ; b ; yushicang@163.com" class="auth_mail" title="E-mail the corresponding author ; Xiu-wu Biana ; b ; bianxiuwu@263.net" class="auth_mail" title="E-mail the corresponding author
- 关键词：Glioma ; Stem cell ; CD133 ; Flow cytometry ; Cell dissociation
- 刊名：Cancer Letters
- 出版年：2016
- 出版时间：10 July 2016
- 年：2016
- 卷：377
- 期：1
- 页码：105-115
- 全文大小：4326 K
文摘
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This is the first study to optimize the dissociation protocol for isolating human glioma stem cells (GSCs) from tumorspheres.
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Liberase-TL (200 µg/ml) is an ideal reagent for isolating GSCs from tumorspheres via fluorescence-activated cell sorting.
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Non-enzymatic cell dissociation solution (NECDS) for such a protocol should be carefully considered.