Development of a custom pentaplex sandwich immunoassay using Protein-G coupled beads for the Luminex® xMAP® platform
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- 作者：Jacqueline U. McDonalda ; jacqueline.mcdonald@imperial.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Julia Ekeruche-Makindea ; Mei M. Hob ; John S. Tregoninga ; 1 ; Omodele Ashirub ; 1 ; Omodele.Ashiru@nibsc.org" class="auth_mail" title="E-mail the corresponding author
- 关键词：xMAP® ; Protein G ; Beads ; Sandwich immunoassay ; Biomarkers ; Serum
- 刊名：Journal of Immunological Methods
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：433
- 期：Complete
- 页码：6-16
- 全文大小：997 K
文摘
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Custom pentaplex array for detection of candidate biomarkers of vaccine safety
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Protein G-coupled beads facilitate the development of custom arrays.
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In-house buffer significantly alleviates serum matrix effects.