Peptide-functionalized ZCIS QDs as fluorescent nanoprobe for targeted HER2-positive breast cancer cells imaging

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• 作者：Martyna Michalska^a ; ^b ; ^c ; Anna Florczak^b ; ^d ; Hanna Dams-Kozlowska^d ; ^e ; Jacek Gapinski^b ; ^f ; Stefan Jurga^b ; ^c ; Raphaë ; l Schneider^a ; ^{raphael.schneider@univ-lorraine.fr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：CuInZnxS2+x quantum dots ; LTVSPWY peptide ; Cancer diagnosis ; Breast cancer ; HER2 targeting ; Fluorescence imaging
• 刊名：Acta Biomaterialia
• 出版年：2016
• 出版时间：15 April 2016
• 年：2016
• 卷：35
• 期：Complete
• 页码：293-304
• 全文大小：3323 K

文摘

In this paper, the synthesis of alloyed CuInZn_xS_2+x quantum dots (ZCIS QDs), their transfer into aqueous solution via a polymer coating technique, and the use of these nanocrystals to selectively target HER2-positive cells, are reported. By optimizing first the ZnS shell deposition process onto the CuInS₂ core, and next the encapsulation of the dots with the amphiphilic poly(maleic anhydride-alt-1-octadecene) (PMAO) polymer, water-dispersible ZCIS QDs were successfully prepared. The nanocrystals with a photoluminescence quantum yield of 35% were purified via centrifugation and ultracentrifugation and high quality nanoparticles with narrow size distributions and surface charges were obtained. After verifying the biocompatibility of PMO-coated ZCIS QDs, we coupled these nanocrystals with the LTVSPWY peptide and demonstrated via MTT assay that both bare and the peptide-linked QDs exhibit low cytotoxicity. The HER2-mediated delivery of the peptide-linked QDs was confirmed by confocal microscopy. This study indicates that as engineered QDs can efficiently be used as fluorescent nanoprobes for selective labelling of HER2-positive SKBR3 cancer cells.