Highly efficient enrichment of porcine cells with deletions induced by CRISPR/Cas9 using dual fluorescence selection
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- 作者：Zuyong He1 ; Xuan Shi1 ; Baozhu Du ; Yufeng Qin ; Peiqing Cong ; Yaosheng Chen ; chyaosh@mail.sysu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：CRISPR/Cas9 ; Genomic deletion ; FACS ; BMP15
- 刊名：Journal of Biotechnology
- 出版年：2015
- 出版时间：20 November 2015
- 年：2015
- 卷：214
- 期：Complete
- 页码：69-74
- 全文大小：2371 K
文摘
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Dual fluorescence selection system can enrich genomic deletions efficiently.
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It can enrich cells with 170 bp small deletions at a frequency as high as 74%.
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It can enrich cells with 5 kb large deletions at a frequency of 46%.