The new immunosuppressant PLNPK prolongs allograft survival in mice

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• 作者：Lijuan Wang ; Song Wang ; Rong Lu ; Junqiang Lv ; Chunlei Zhou ; Zheng Fu ; Qiong Xu ; Xuchun Che ; Jing Jia ; Hui Zhao ; Xueying Li ; Gang Lin ; Zhi Yao
• 关键词：PLNPK ; Skin transplantation ; Cardiac transplantation ; IL-2
• 刊名：Transplant Immunology
• 出版年：2010
• 出版时间：October 2010
• 年：2010
• 卷：24
• 期：1
• 页码：64-68
• 全文大小：874 K

文摘

The pentapeptide PLNPK (Pro-Leu-Asn-Pro-Lys) is extracted from the spleen. Preliminary studies have shown that PLNPK could inhibit T lymphocyte transformation and antibody production. In the present study, we detected the inhibitory effect of PLNPK on one-way mixed leukocyte reaction (MLR) in vitro and observed the effect of PLNPK on the duration of allograft survival in mouse models of skin or cardiac transplantation. Pathological damage and T cell infiltration of the grafts were also detected. Results showed that PLNPK could significantly inhibit T lymphocyte proliferation, with an optimized inhibition of 40 % . Also PLNPK could significantly prolong the mean survival time of skin allograft and cardiac allograft, producing survival rates of 42 % and 38.7 % , respectively. PLNPK at a dose of 200 μg/kg/d or 100 μg/kg/d could significantly suppress ConA-induced T cell proliferation and T cell IL-2 secretion in transplant recipient mice, compared to the saline group (P < 0.05). This information suggests that PLNPK can effectively antagonize transplant rejection, possibly by reducing IL-2 secretion by T cells and inhibiting T cell proliferation and activation.