Identification of Salmonella enterica Typhimurium and variants using a novel multiplex PCR assay
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- 作者：Xiaohua Hea ; Xuebin Xub ; Ke Lic ; Bin Liud ; liubin7723@nwsuaf.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Tianli Yued
- 关键词：Salmonella Typhimurium ; Variant ; MLST ; Antibiotic resistance ; Multiplex PCR
- 刊名：Food Control
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：65
- 期：Complete
- 页码：152-159
- 全文大小：714 K
文摘
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Genes STM4495, oafA, fliC, and fljB as targets.
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One primer set was selected for each gene.
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A novel multiplex PCR method was developed.
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This PCR assay was specific and sensitive for detecting S. Typhimurium and variants.
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All 1,4,[5],12:i:- strains were identified as ST34 with multidrug resistances.