Toxoplasma gondii
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- 作者：Houshuang Zhang ; Oriel M.M. Thekisoe ; Gabriel O. Aboge ; Hisako Kyan ; Junya Yamagishi ; Noboru Inoue ; Yoshifumi Nishikawa ; Satoshi Zakimi ; Xuenan Xuan
- 关键词：Toxoplasma gondii ; Loop-mediated isothermal amplification (LAMP) ; 529 bp gene
- 刊名：Experimental Parasitology
- 出版年：2009
- 出版时间：May 2009
- 年：2009
- 卷：122
- 期：1
- 页码：47-50
- 全文大小：207 K
文摘
Loop-mediated isothermal amplification (LAMP) method amplifies DNA with high specificity, sensitivity and rapidity. In this study, we used a conserved sequence in the 200- to 300-fold repetitive 529 bp gene of Toxoplasma gondii to design primers for LAMP test. Detection limit of T. gondii LAMP assay with the primers is 1 pg/μL of T. gondii DNA, which was evaluated using 10-fold serially diluted DNA of cultured parasites. Furthermore, LAMP and conventional PCR methods were applied for amplification of the T. gondii DNA extracted from the lymph nodes taken from pigs which were suspected to be Toxoplasma infection. As a result, 76.9 % (70/91) and 85.7 % (78/91) of the samples were positive on PCR and LAMP analyzes, respectively. Therefore, the LAMP has a potential to be applied as an alternative molecular diagnostic tool for detection of T. gondii infection from veterinary samples. This is the first study, which applies the LAMP method to diagnose Toxoplasma from veterinary samples.