Tailoring of global transcription sigma D factor by random mutagenesis to improve Escherichia coli tolerance towards low-pHs
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- 作者：Xi Gaoa ; melindag@yeah.net" class="auth_mail" title="E-mail the corresponding author ; Ling Jiangb ; jiangling@njtech.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Liying Zhuc ; zlyhappy@njtech.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Qing Xua ; xuqing357@njtech.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Xian Xua ; xuxian@njtech.edu.cn" class="auth_mail" title="E-mail the corresponding author ; He Huanga ; biotech@njtech.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Escherichia coli ; Acid tolerance ; RpoD ; RNA-seq ; Phenotype microarray
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：20 April 2016
- 年：2016
- 卷：224
- 期：Complete
- 页码：55-63
- 全文大小：2610 K
文摘
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RAISE was first used to engineer the global regulator RpoD of E. coli to improve its acid tolerance.
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Best mutant was obtained through multiwheel of acid-shock screening with significantly improved growth rate at pH 3.17.
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Combined transcriptome and phenome analysis of E. coli was carried out to better understand the global effects of RpoD on the regulatory networks.
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RpoD factor can regulate both the master regulators and gene/operon-specific transcription factors.