Improving special hydrolysis characterization into Talaromyces thermophilus F1208 xylanase by engineering of N-terminal extension and site-directed mutagenesis in C-terminal

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• 作者：Qin Li^a ; ^b ; Baoguo Sun^a ; ^b ; Ke Xiong^b ; Chao Teng^c ; Youqiang Xu^b ; ^c ; Liangjun Li^b ; Xiuting Li^a ; ^b ; ^{lixt@btbu.edu.cn} ; ^{li_xiuting@163.com}
• 关键词：Xylanase ; Hydrolysis ; Xylo-oligosaccharides ; Protein engineering
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：96
• 期：Complete
• 页码：451-458
• 全文大小：1841 K
• 卷排序：96

文摘

Xylanase T-Xyn has a long suspected N-terminal extension. Hybrid xylanase T-XynFM showed special hydrolysis characterization. No xylose produced when hydrolyzed xylotriose, xylotetraose and xylopentaose. Modulating hydrolysis and transglycosylation activity by protein engineering.