Expression pattern of cellulolytic and xylanolytic genes regulated by transcriptional factors XYR1 and CRE1 are affected by carbon source in Trichoderma reesei

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• 作者：Lilian dos Santos Castro^a ; ¹ ; Amanda Cristina Campos Antoniê ; to^a ; ¹ ; Wellington Ramos Pedersoli^a ; Rafael Silva-Rocha^a ; Gabriela F. Persinoti^b ; Roberto Nascimento Silva^a ; ^{rsilva@fmrp.usp.br" class="auth_mail}
• 关键词：Cellulases ; Gene expression ; Transcriptional factor ; Trichoderma reesei ; Xylanases
• 刊名：Gene Expression Patterns
• 出版年：March, 2014
• 年：2014
• 卷：14
• 期：2
• 页码：88-95
• 全文大小：1389 K

文摘

Trichoderma reesei is the most important fungus for the industrial production of enzymes to biomass deconstruction. Most of the genes encoding cellulases and hemicellulases are regulated by the transcription factors CRE1 and XYR1. In this work, the regulation of 22 genes of cellulases and xylanases by these transcription factors was investigated under three different carbon sources. Analysis of gene expression and enzymatic profiles of CMCase, 尾-glucosidase, and xylanases showed different regulation that was depended of the carbon source in both 螖xyr1 and 螖cre1 mutants. In the presence of glucose, the majority of genes evaluated (82%) showed increased expression levels in the 螖cre1 mutant compared to the parental QM9414 strain. In the 螖xyr1 mutant, it was observed that expression of cellulase and xylanase genes was reduced compared to the parental QM9414 strain, when cultured in the presence of cellulose or sophorose. Interesting, in the presence of glucose, approximately 60% of the analyzed genes had increased expression in the 螖xyr1 mutant compared to parental strain. Furthermore, no correlation between gene expression and the number of putative binding sites of XYR1 and CRE1 to promoter region of cellulolytic and xylanolytic studied genes was observed. Therefore, these results demonstrated that the regulation of cellulase and xylanase by the transcription factors CRE1 and XYR1 is influenced by different carbon sources.