Cloning and high-level expression of β-xylosidase from Selenomonas ruminantium in Pichia pastoris by optimizing of pH, methanol concentration and temperature conditions
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- 作者：Ehsan Dehnavia ; Seyed Omid Ranaei Siadatb ; c ; o_ranaei@sbu.ac.ir" class="auth_mail" title="E-mail the corresponding author ; Mehrnoosh Fathi Roudsarid ; Khosro Khajeha ; khajeh@modares.ac.ir" class="auth_mail" title="E-mail the corresponding author
- 关键词：β-xylosidase ; Pichia pastoris ; Heterologous expression ; Optimization
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：124
- 期：Complete
- 页码：55-61
- 全文大小：704 K
文摘
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β-xylosidase from Selenomonas ruminatium was expressed in Pichia pastoris using alcohol oxidase I promoter.
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The synthetic gene was optimized based on Pichia pastoris codon preference.
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This is the first report on the cloning and expression of a β-xylosidase gene from GH family 43 in Pichia pastoris.
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Expression of β-xylosidase was increased three times by optimization of growth and induction conditions in shake flask.
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The recombinant xylosidase showed higher turnover rate rather than most xylosidase reported previously.