Aldehyde-stabilized cryopreservation
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- 作者：Robert L. McIntyre ; rlm@21cm.com" class="auth_mail" title="E-mail the corresponding author ; rlm@mit.edu" class="auth_mail" title="E-mail the corresponding author ; Gregory M. Fahy gfahy@21cm.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Vitrification ; Connectomics ; Brain banking ; Perfusion ; Fixation ; Electron microscopy ; Cryopreservation ; Neuroanatomy ; Ultrastructure ; Pig ; Rabbit ; Cryoprotective agents ; Long term storage
- 刊名：Cryobiology
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：71
- 期：3
- 页码：448-458
- 全文大小：5042 K
文摘
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We introduce aldehyde-stabilized cryopreservation (ASC), a brain-banking technique for preserving detailed brain ultrastructure over long time scales.
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ASC uses glutaraldehyde to rapidly stabilize brain ultrastructure.
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ASC uses vitrification to preserve brains over indefinite time scales.
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Fixative/cryoprotectants are delivered via perfusion, enabling preservation of brains of any size.
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Cryoprotectants can be removed either by diffusion from slices or via perfusion, yielding a fixed brain compatible with many connectomics assays.