The Histone Modification Domain of Paf1 Complex Subunit Rtf1 Directly Stimulates H2B Ubiquitylation through an Interaction with Rad6
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- 作者：S. Branden Van Oss1 ; 7 ; Margaret K. Shirra1 ; 7 ; Alain R. Bataille2 ; 7 ; Adam D. Wier1 ; Kuangyu Yen2 ; 3 ; Vinesh Vinayachandran2 ; In-Ja L. Byeon4 ; Christine E. Cucinotta1 ; Annie Hé ; roux5 ; Jongcheol Jeon6 ; Jaehoon Kim6 ; Andrew P. VanDemark1 ; B. Franklin Pugh2 ; Karen M. Arndt1 ; 8 ; arndt@pitt.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Paf1 complex ; H2B ubiquitylation ; RNA Polymerase II ; histone modifications ; transcription elongation ; Rtf1 ; Rad6 ; Bre1 ; chromatin
- 刊名：Molecular Cell
- 出版年：2016
- 出版时间：17 November 2016
- 年：2016
- 卷：64
- 期：4
- 页码：815-825
- 全文大小：3071 K
文摘
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Histone modification domain (HMD) of Rtf1 directly contacts Rad6 in vivo
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HMD structure and in vivo crosslinking identify a conserved Rad6 interaction surface
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Paf1C regulates global H2B ubiquitylation (ub) patterns by linking HMD to Pol II
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HMD stimulates Bre1-dependent H2Bub in a transcription-free, reconstituted assay