A novel ELISA using a recombinant outer membrane protein, rTp0663, as the antigen for serological diagnosis of syphilis
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- 作者：Man Xua ; b ; c ; 1 ; Yafeng Xiea ; b ; c ; 1 ; Chuanhao Jianga ; b ; c ; 1 ; Yongjian Xiaoa ; b ; c ; d ; Xingxing Kuanga ; b ; c ; Feijun Zhaoa ; b ; c ; Tiebing Zenga ; b ; c ; Shuangquan Liue ; Mingxing Lianga ; b ; c ; Li Lia ; b ; c ; Chuan Wanga ; b ; c ; Yimou Wua ; b ; c ; yimouwu@sina.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Treponema pallidum ; Syphilis ; Serodiagnosis ; Recombinant antigen ; Tp0663 ; ELISA
- 刊名：International Journal of Infectious Diseases
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：43
- 期：Complete
- 页码：51-57
- 全文大小：599 K
文摘
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A soluble recombinant version of a new Treponema pallidum outer membrane protein, known as Tp0663, has been produced for the diagnosis of syphilis.
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The recombinant protein exhibited a superior serodiagnostic performance to the ARCHITECT Syphilis TP chemiluminescent immunoassay (Abbott GmbH & Co. KG) when 3326 characterized serum samples were screened with the two tests.
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The Tp0663-based ELISA was in perfect agreement with clinical diagnosis (gold standard) with a κ value of 0.99, comparable to that of ARCHITECT Syphilis TP (0.96).
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Tp0663 may represent a novel candidate with highly accurate diagnosis for the seroscreening of syphilis.