Leucine promotes glucose uptake in skeletal muscles of rats

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• 作者：Nishitani ; Shinobu ; Matsumura ; Tsuyoshi ; Fujitani ; Shoji ; Sonaka ; Ichiro ; Miura ; Yutaka ; Yagasaki ; Kazumi
• 关键词：Leucine ; Insulin ; α ; -KIC ; Glucose uptake ; Soleus ; PI3-kinase ; PKC ; mTOR
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2002
• 出版时间：December 20, 2002
• 年：2002
• 卷：299
• 期：5
• 页码：693-696
• 全文大小：138 K

文摘

Soleus muscles isolated from normal rats were incubated to evaluate whether or not leucine promotes glucose uptake under insulin-free conditions, using a labeled 2-deoxyglucose uptake assay. Glucose uptake was promoted by 2mM leucine. A metabolite of leucine, α-ketoisocaproic acid (α-KIC), also exhibited a similar stimulatory effect, although this was not as potent as leucine. Stimulation of glucose uptake by leucine was completely canceled by pre-treatment with either 10μM LY294002, a specific inhibitor of phosphatidylinositol 3-kinase (PI3-kinase), or 6μM GF109203X, a specific inhibitor of protein kinase C (PKC). No significant change was observed by pre-treatment with 1μM rapamycin, a specific inhibitor of mammalian target of rapamycin (mTOR). These results suggest that leucine stimulates glucose transport in skeletal muscle via PI3-kinase and PKC pathways independently of the mammalian target of mTOR. They also suggest that leucine stimulates glucose transport by an insulin-independent mechanism.