Poly[(9,9-dioctyl-fluorenyl-2,7-diyl)-co-fluorenone]-based orange fluorescence probe for cellular imaging

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• 作者：Yuan Liu^a ; Hua Wang^a ; ^c ; ^{wanghua001@tyut.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Peng Tao^a ; Xing Liu^a ; Yahong Liu^b ; Qingchen Dong^a ; Wenqing Liang^a ; Qiang Zhao^b ; Wai-Yeung Wong^c ; ^{rwywong@hkbu.edu.hk" class="auth_mail" title="E-mail the corresponding author} ; Bingshe Xu^a
• 关键词：Poly[(9 ; 9-dioctyl-fluorenyl-2 ; 7-diyl)-co-fluorenone] ; Orange fluorescence probe ; Cellular imaging ; Rhodamine B
• 刊名：Tetrahedron
• 出版年：2016
• 出版时间：5 May 2016
• 年：2016
• 卷：72
• 期：18
• 页码：2287-2292
• 全文大小：974 K

文摘

A novel orange fluorescence probe based on efficient fluorescence resonance energy transfer from poly[(9,9-dioctylfluorenyl-2,7-diyl)-co-fluorenone] nanoparticles to Rhodamine B dye molecules was developed. This novel probe exhibited a larger Stokes shift, enhanced photostability and longer lifetime comparing to Rhodamine B dye molecules. Meanwhile, it demonstrated the characteristics that especially useful in live cell or in vivo studies, such as narrow full width at half-maximum emission, excitation bands in the visible range and the sensitivity of its fluorescence to temperature. Confocal fluorescence images of the fluorescent probe proved that it could effectively label HeLa cells.