Generation of human iPSCs from urine derived cells of a non-affected control subject

详细信息    查看全文

• 作者：Muhammad Zeeshan Afzal^a ; Melanie Gartz^a ; Ekaterina A. Klyachko^b ; Sadiya Sana Khan^b ; Sanjiv J. Shah^b ; Sweta Gupta^c ; Amy D. Shapiro^c ; Douglas E. Vaughan^b ; Jennifer L. Strande^a ;
• 刊名：Stem Cell Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：18
• 期：Complete
• 页码：33-36
• 全文大小：825 K
• 卷排序：18

文摘

We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from non-affected control subjects to use as a comparison group for the iPSC lines containing a Plasminogen Activator Inhibitor-1 (PAI-1 homozygous/heterozygous) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the UCs.