- 作者：Nozomu Yamauchi ; DDS&lowast ; ; noz.yamauchi@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Shizuko Yamauchi ; DDS ; MS&lowast ; ; Hideaki Nagaoka ; DDS ; PhD&dagger ; ; Derek Duggan ; DDS ; MS&lowast ; ; Sheng Zhong ; DDS&lowast ; ; Sun Min Lee ; BA&dagger ; ; Fabricio B. Teixeira ; DDS ; MS ; PhD&Dagger ; ; Mitsuo Yamauchi ; DDS ; PhD&dagger ;
- 关键词：Regenerative endodontics ; revascularization ; root canal ; tissue engineering
- 刊名：Journal of Endodontics
- 出版年：2011
- 出版时间：March 2011
- 年：2011
- 卷：37
- 期：3
- 页码：390-397
- 全文大小：1089 K
Methods
Apical periodontitis was induced in 6 dogs with immature teeth (n = 64). After disinfection, the following groups were randomly assigned: blood clot (BC) alone, BC with a cross-linked collagen scaffold (CCS), BC with exposure of dentin matrix by ethylenediaminetetraacetic acid (EDTA), and BC with CCS and EDTA. Positive (infected only) and negative controls (untreated) were also included. The dogs were followed up for 3.5 months and killed. Periradicular healing and root wall thickening were radiographically analyzed and statistically evaluated. The jaws were then fixed, demineralized, and subjected to histologic analyses. Newly formed mineralized tissues were histomorphometrically analyzed, quantified, and statistically evaluated.
Results
Radiographically there was significant difference in periradicular healing and root wall thickening (P < .05). Histomorphometric analysis showed significantly more mineralized tissue formation in the groups containing the scaffold (P < .05). Exposure of the dentin matrix by EDTA appeared to increase the adherence of the newly formed mineralized tissue to the root walls.
Conclusions
The use of cross-linked collagen scaffold and exposure of dentin matrix combined with blood clot might provide an efficient approach to generate a vital support structure for the treatment of immature teeth with apical periodontitis.