Graphene quantum dots as additives in capillary electrophoresis for separation cinnamic acid and its derivatives

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• 作者：Yaming Sun^a ; ^b ; Qing Bi^c ; Xiaoli Zhang^a ; ^b ; Litao Wang^a ; Xia Zhang^a ; Shuqing Dong^a ; ^{sqdong@licp.cas.cn" class="auth_mail" title="E-mail the corresponding author} ; Liang Zhao^a ; ^{zhaol@licp.cas.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Graphene quantum dots ; Additives ; Capillary electrophoresis ; Cinnamic acid and its derivatives
• 刊名：Analytical Biochemistry
• 出版年：2016
• 出版时间：1 May 2016
• 年：2016
• 卷：500
• 期：Complete
• 页码：38-44
• 全文大小：1502 K

文摘

A facile capillary electrophoresis (CE) method for the separation of cinnamic acid and its derivatives (3,4-dimethoxycinnamic acid, 4-methoxycinnamic acid, isoferulic acid, sinapic acid, cinnamic acid, ferulic acid, and trans-4-hydroxycinnamic acid) using graphene quantum dots (GQDs) as additives with direct ultraviolet (UV) detection is reported. GQDs were synthesized by chemical oxidization and further purified by a macroporous resin column to remove salts (Na₂SO₄ and NaNO₃) and other impurities. Transmission electron microscopy (TEM) indicated that GQDs have a relatively uniform particle size (2.3 nm). Taking into account the structural features of GQDs, cinnamic acid and its derivatives were adopted as model compounds to investigate whether GQDs can be used to improve CE separations. The separation performance of GQDs used as additives in CE was studied through variations of pH, concentration of the background electrolyte (BGE), and contents of GQDs. The results indicated that excellent separation can be achieved in less than 18 min, which is mainly attributed to the interaction between the analytes and GQDs, especially isoferulic acid, sinapic acid, and cinnamic acid.