Overexpression of Peroxiredoxin 6 Protects Neoplastic Cells against Apoptosis in Canine Haemangiosarcoma

详细信息    查看全文

• 作者：Sh. Anwarup>&lowast ; up>up> ; up>up>&dagger ; up> ; T. Yanaiup>&lowast ; up> ; H. Sakaiup>&lowast ; up>up> ; up>up>&Dagger ; up>up> ; up>up>shiroki@gifu-u.ac.jp" class="auth_mail" title="E-mail the corresponding authorup>
• 关键词：apoptosis ; dog ; haemangiosarcoma ; peroxiredoxin 6
• 刊名：Journal of Comparative Pathology
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：155
• 期：1
• 页码：29-39
• 全文大小：1625 K

文摘

Canine haemangiosarcoma (HSA), like human angiosarcoma, is an uncommon malignant vascular endothelial cell tumour associated with a poor prognosis. The peroxiredoxin (PRDX) family of peroxidases, which comprises six members in mammals (PRDX1–6), might contribute to cancer cell survival in the face of oxidative stress as these proteins exhibit frequent upregulation in cancer cells. In this study, we investigated the expression levels of PRDX6 in spontaneously arising primary canine HSAs by immunohistochemical analysis, identifying marked expression of this protein. Both PRDX6 mRNA and protein were overexpressed in HSA cell lines compared with normal canine endothelial cells, although some variation was observed between the different HSA cell lines. Small interfering RNA-induced downregulation of PRDX6 promoted apoptosis in the HSA cell lines. The observation that PRDX6 suppression increased the cytotoxicity of these cells suggests that PRDX6 might play an important cytoprotective role.