We cloned a putative cDNA of α--rhamnosidase from Aspergillus niger JMU-TS528 and extracellular over-expressed this cDNA in Pichia pastoris GS115.
The activity of the recombinant α--rhamnosidase r-Rha1 is eightfold higher than the native enzyme.
r-Rha1 is a N-glycosylated protein possessed broad substrate specificities, a good thermostability, wide range of pH-stability, and excellent tolerance abilities against glucose and ethanol.