Phage display-derived oligopeptide-functionalized probes for in vivo specific photoacoustic imaging of osteosarcoma

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• 作者：Zebin Ma ; MMed^a ; ¹ ; Huan Qin ; PhD^b ; ¹ ; Hongjiang Chen ; MMed^a ; Hailong Yang ; MMed^a ; Jiankun Xu ; PhD^a ; Sihua Yang ; PhD^b ; Jun Hu ; PhD^a ; ^{hjzkm@vip.163.com} ; Da Xing ; PhD^b ; ^{xingda@scnu.edu.cn}
• 关键词：Osteosarcoma ; Phage display ; Oligopeptides ; Photoacoustic imaging
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：13
• 期：1
• 页码：111-121
• 全文大小：2090 K
• 卷排序：13

文摘

Specific detection of various tumor types remains crucial for designing effective treatment strategies. We demonstrate photoacoustic imaging (PAI) using high-affinity and high-specificity peptide-based probes for accurate and specific diagnosis of osteosarcoma. Herein, two new tumor-specific oligopeptides, termed PT6 and PT7, were identified using phage display-based screening on an osteosarcoma cell line (UMR-106). The identified oligopeptides were able to detect clinical osteosarcoma samples on tissue microarrays. Oligopeptide-conjugated PEGylated gold nanorods (PGNR) were designed to specifically target UMR-106 cells. More importantly, PAI revealed that both PGNR-PT6 and PGNR-PT7 could bind selectively to subcutaneous UMR-106 xenografts after systemic administration and enhance the contrast of osteosarcoma images by 170% and 230%, respectively, compared to tumor-bearing mice injected with PGNRs conjugated to scrambled oligopeptides. PAI employing PGNRs conjugated to specifically designed nanoprobes may provide a new method for tumor type-specific diagnosis of osteosarcoma.