Non-covalent decoration of carbon dots with folic acid via a polymer-assisted strategy for fast and targeted cancer cell fluorescence imaging

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• 作者：Dan LeiAuthor Vitae ; Wen Yang ; ^{wenyang@bit.edu.cn" class="auth_mail" title="E-mail the corresponding author}Author Vitae ; Yunqian GongAuthor Vitae ; Jing JingAuthor Vitae ; Hailiang NieAuthor Vitae ; Bin YuAuthor Vitae ; Xiaoling Zhang ; ^{zhangxl@bit.edu.cn" class="auth_mail" title="E-mail the corresponding author}Author Vitae
• 关键词：Non-covalent decoration ; Carbon dots ; Folate acid ; Folate receptor-positive cancer cells ; Turn on fluorescent nanoprobe
• 刊名：Sensors & Actuators: B. Chemical
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：230
• 期：Complete
• 页码：714-720
• 全文大小：1997 K

文摘

An efficient approach for targeting and detecting folate-receptor (FR)-positive cancer cells was developed through non-covalent conjugation of folic acid (FA) on polyethyleneimine modified CDs (PEI-CDs). The fluorescent CDs were prepared by a facile hydrothermal method, and their surfaces were wrapped with positively charged PEI for further conjugation with FA through electrostatic interaction. The FA targeted PEI modified CDs (FA-PEI-CDs) can be used as a turn-on fluorescent nanoprobe for folate receptor (FR)-positive cancer cells in vivo and in vitro. The uptake of the designed FA-PEI-CDs by HeLa and HepG2 cancer cells was verified by confocal laser scanning microscopy after 10 min incubation and competition experiments, as well as a comparative study with FR-negative MCF-7 cells.