Induction of yeast apoptosis by an antimicrobial peptide, Papiliocin

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• 作者：Bomi Hwang^a ; ¹ ; Jae-Sam Hwang^b ; ¹ ; Juneyoung Lee^a ; Jin-Kyoung Kim^c ; Seong Ryul Kim^b ; Yangmee Kim^c ; Dong Gun Lee^a ; ^{dglee222@knu.ac.kr"" rel=""nofollow}
• 关键词：Papiliocin ; Antimicrobial peptide ; Apoptosis ; Candida albicans ; Papilio xuthus
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2011
• 出版时间：29 April 2011
• 年：2011
• 卷：408
• 期：1
• 页码：89-93
• 全文大小：699 K

文摘

Papiliocin is a 37-residue peptide isolated from the swallowtail butterfly Papilio xuthus. In this study, we found that Papiliocin induced the accumulation of reactive oxygen species (ROS) and hydroxyl radicals known to be important regulators of apoptosis in Candida albicans. To examine the relationship between the accumulation of ROS and the induction of apoptosis, we investigated the apoptotic effects of Papiliocin using apoptotic markers. Cells treated with Papiliocin showed a series of cellular changes normally seen in cells undergoing apoptosis: plasma membrane translocation of phosphatidylserine from the inner to the outer membrane leaflet, measured by Annexin V staining, dissipation of the mitochondrial membrane potential, observed by DiOC₆(3) staining; and the presence of active metacaspases, measured using the CaspACE FITC-VAD-FMK, as early apoptotic events. In addition, DNA condensation and fragmentation, which is important marker of late stage apoptosis, was seen by DAPI and TUNEL assay. Therefore, these results suggest that Papiliocin leads to apoptosis in C. albicans via ROS accumulation.